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Blood, Vol. 113, Issue 12, 2835-2842, March 19, 2009
Platelets actively sequester angiogenesis regulators
Blood Lakka Klement et al.
113: 2835
Supplemental materials for: Lakka Klement et al
Files in this Data Supplement:
- Figure S1. Semi-quantitative evaluation of platelet protein profiles of tumor-bearing and non-tumor bearing mice (JPG, 72.9 KB)
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Presented are four angiogenesis regulators and their respective patterns of expression. At 30 days post implantation of the tumors, the levels of pro-angiogenic growth factors, such VEGF, bFGF and PDGF, were elevated in the platelets of tumor-bearing mice as compared to tumor-free sham operated mice. The levels of these proteins in platelets of non-tumor-bearing mice were much lower and comparable to those in plasma. Despite the small size of the nonangiogenic tumors (~100 times smaller than their angiogenic counterparts) there are detectable differences in the levels of angiogenesis regulators as compared platelets of control animals. In the mice bearing nonangiogenic tumors, the elevation of positive angiogenesis regulators tended to be counterbalanced by an elevation of the endogenous inhibitor endostatin, whereas in mice bearing the angiogenic clone (red) platelets showed a decrease in endostatin. This suggests that, in the “angiogenic” tumors, the overall balance of angiogenesis regulators may be tipped towards a more pro-angiogenic phenotype in a manner reflected on the platelet proteome. The experiment was repeated on two separate occasions with 5 mice per each experiment, and the graph represents means ± standard error of means.
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