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Blood, Vol. 113, Issue 25, 6277-6287, June 18, 2009 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Expansion of peripheral naturally occurring T regulatory cells by Fms-like tyrosine kinase 3 ligand treatment Blood Swee et al. 113: 6277 Supplemental materials for: Swee et al Files in this Data Supplement: Figure S1. NTreg and CD11c+ splenic population go back to normal values after FLT3L treatment arrest (JPG, 23.1 KB) - C57Bl/6 mice were treated with FLT3L 10µg intraperitonal injection daily from day 1 to 10 or left untreated. NTreg and DC population were analysed in the spleen at day 10, 20 or 30. (A) Percentage of Foxp3+ cells amongst CD4 T cells in the spleen of control or treated mice. (B) Percentages of CD11c+ cells in the spleen of control or treated mice. Each Bars shows mean +∕− standard error of the mean. *, p<0.05; **, p<0.01. Figure S2. FLT3L does not induce proliferation of sorted NTreg in vitro (JPG, 39.3 KB) - PKH26-labeled CD4+Foxp3+ cell sorted from Foxp3EGFP reporter mice were stimulated in medium alone or supplemented with IL-2 or FLT3L supernatant in presence or absence of plate-bound anti-CD3. Histograms show PHK26 level on CD4+Foxp3+ cells after 4 days of incubation. Figure S3. Sorted NTreg proliferate in presence of GM-CSF–differentiated BMDC (JPG, 34.3 KB) - PKH26-labeled CD4+Foxp3+ cell sorted from Foxp3EGFP reporter mice were incubated with FLT3L- or GM-CSF–derived BMDC (filled histograms) or alone (empty histogram) in presence of IL-2 supernatant. Histograms show PHK26 level on CD4+Foxp3+ cells after 5 days of incubation. Figure S4. FLT3L treatment prevents mice dying from by acute Graft versus Host disease (JPG, 26.4 KB) - (C57Bl/6 × DBA/2)F1 were injected with 50 × 106 pooled Lymphnode/spleen from C57Bl/6 mice. Recipient mice were either kept as control or treated with FLT3L one week after GvHD induction. Curves show percentage survival of each group. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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