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Blood, Vol. 113, Issue 3, 755-764, January 15, 2009
Critical role of scavenger receptor-BI–expressing bone marrow–derived endothelial progenitor cells in the attenuation of allograft vasculopathy after human apo A-I transfer
Blood Feng et al.
113: 755
Supplemental materials for: Feng et al
Files in this Data Supplement:
- Document 1. Supplemental materials, methods, and results (PDF, 75.8 KB)
- Figure S1. SR-BI expression in cultured human endothelial progenitor cells (EPCs) (JPG, 55.4 KB)
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Mononuclear cells were isolated from peripheral blood and cultivated in EGM-2MV BulletKit medium for 4 days. (A) Human EPCs stained with Dil-acLDL (red); (B) Human EPCs stained with SR-BI antibody (green); (C) Merged view of Dil-acLDL and SR-BI double positive cells. Scale bar represents 100 µm.
- Figure S2 (JPG, 77.5 KB)
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Cholesterol lipoprotein profiles obtained by fast performance liquid chromatography gel filtration of 100 µl plasma obtained at day 0 (F), day 14 (E), and day 35 (H) after gene transfer with AdA-I or Adnull in C57BL/6 mice 4 weeks after transplantation with SR-BI+∕+ or SR-BI−∕− bone marrow. Fractions 12–32 correspond to non-HDL sized particles whereas fractions 33–45 correspond to HDL sized particles.
- Figure S3. SR-BI dependence of HDL-induced EPC migration (JPG, 57.2 KB)
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Bar graph showing the number of migrated EPCs in modified Boyden chambers. After 7 days of culture, Sca-1 positive bone marrow EPCs isolated from wild-type or SR-BI−∕− mice were seeded in the upper chamber. The lower chamber was supplemented with either HDL (100 µg/ml) or an equivalent amount of bovine serum albumin and the number of migrated cells per microscopy field was quantified after 5 hours (n=4 for each group).
- Figure S4. Effect of human apo A-I transfer on macrophage infiltration in the intima of SR-BI+∕+ allografts of chimeric C57BL/6 mice and chimeric C57BL/6 SR-BI−∕− mice (JPG, 129 KB)
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Representative sections of Mac-3 positive macrophages at day 21 after transplantation in control (A, C) and AdA-I (B, D) treated C57BL/6 mice with SR-BI+∕+ (A–B) or SR-BI−∕−(C–D) bone marrow. Scalebar represents 100 µm.
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