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Blood, Vol. 113, Issue 6, 1278-1286, February 5, 2009
Cell adhesion through  V-containing integrins is required for efficient HIV-1 infection in macrophages
Blood Ballana et al.
113: 1278
Supplemental materials for: Ballana et al
Files in this Data Supplement:
- Figure S8. HIV-1 replication in MDM on vitronectin-coated plates (JPG, 43.1 KB)
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MDM of six different donors were plated onto vitronectin-coated plates and infected with R5 strain HIV-1 BaL. Bars indicate relative p24 production seven days post-infection of MDM coated at indicated vitronectin concentrations, expressed in µg/ml. ND, no drug; VN, vitronectin.
- Figure S9. Effect of soluble vitronectin on HIV-1 replication (JPG, 23.5 KB)
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MDM were infected with R5 HIV-1 strain BaL. Bars indicate relative p24 production seven days post-infection of MDM at indicated S36578 and/or vitronectin concentrations, expressed in µg/ml. Data are the mean±SD of three independent experiments. ND, no drug; VN, vitronectin.
- Figure S10. HIV-1 entry or integration was not blocked by S36578-2 in HeLa cells (JPG, 26.9 KB)
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Proviral DNA of HIV-1 was measured by qRT-PCR in HeLa cells at 8 hours (black bars) and 24 hours post-infection (white bars), in this case preceded by a nested-PCR of Alu-LTR (see methods). Number of HIV-1 copies/cell was obtained using a standard curve and quantification of the cellular gene RNAseP and expressed as percentage compared to the mock-treated cells. For HIV-1 proviral DNA quantification, mean±SD of three independent experiments is shown. One representative experiment is shown for integration assay.
- Figure S11. S36578 did not inhibit GFP expression under the control of GFP promoter (JPG, 52.1 KB)
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Flow cytometry histograms showing the GFP expression in transfected cells with plasmid (green line) compared to mock transfected cells (grey filled lines) and treated with different concentrations of S36578.
- Figure S12. S36578 did not inhibit HIV-1 transcription in HeLa cells cultured in non-adhesive conditions (JPG, 20.7 KB)
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Cells transfected with Tat expressing plasmid were cultured under adhesive and non-adhesive conditions. Absolute values of beta-galactosidase assay (LTR Tat-mediated transactivation) in HeLa cells cultured under adhesive (black bars) or non-adhesive (white bars) conditions 5 days after HIV-1 infection. A representative experiment is shown.
- Figure S13. U0126, an inhibitor of ERK1/2 MAPK pathway inhibited HIV-1 replication in MDM (JPG, 21.1 KB)
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MDM were infected with R5 HIV-1 strain BaL. Black bars indicate relative p24 production seven days post-HIV infection of MDM treated with U0126. Relative cell viability measured by MTT assay is shown in white bars. ND, no drug; NS, no stimulus.
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