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Blood, Vol. 113, Issue 12, 2723-2731, March 19, 2009
VEGF-mediated cross-talk within the neonatal murine thymus
Blood Cuddihy et al.
113: 2723
Supplemental materials for: Cuddihy et al
Files in this Data Supplement:
- Figure S1. Neonate (upper panels) and adult (lower panels) mice were injected in vivo with Cy-3 labelled tomato lectin (JPG, 138 KB)
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After fixation in 4% PFA, thymi were embedded in 3% agarose and cut into 200 µm thick slices. Slices were stained with DAPI and examined on a Zeiss 710 LSM microscope using ECplan-neufluor 10× and 20× objectives scanning to a depth of 50 µm and 20 µm, respectively. The figures shown were assembled from a series of 4 × 3 (10×) or 2 × 2 (20×) tiles using Zeiss Zen software.
- Video 1. 3-D reconstruction of neonatal mouse thymic vasculature (AVI, 1.75 MB)
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Mice were injected in vivo with Cy-3 labelled tomato lectin. After fixation in 4% PFA, thymi were embedded in 3% agarose and cut into 200 µm thick slices. Sections were examined for lectin labelling using a Leica TCS SP1 confocal microscope with a Plan Apo 10×/0.4NA objective and images were acquired using Leica LCS 2.5 software. For each 200 µm section, 20 Z-sections of 6.5 µm each were captured. MetaMorph software (Universal Imaging Corp.) was used to stack the z-sections to provide the 3-D reconstruction.
- Video 2. 3-D reconstruction of adult mouse thymic vasculature (AVI, 1.20 MB)
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Mice were injected in vivo with Cy-3 labelled tomato lectin. After fixation in 4% PFA, thymi were embedded in 3% agarose and cut into 200 µm thick slices. Sections were examined for lectin labelling using a Leica TCS SP1 confocal microscope with a Plan Apo 10×/0.4NA objective and images were acquired using Leica LCS 2.5 software. For each 200 µm section, 20 Z-sections of 6.5 µm each were captured. MetaMorph software (Universal Imaging Corp.) was used to stack the z-sections to provide the 3-D reconstruction.
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