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Blood, Vol. 113, Issue 6, 1365-1374, February 5, 2009
In vitro–differentiated TH17 cells mediate lethal acute graft-versus-host disease with severe cutaneous and pulmonary pathologic manifestations
Blood Carlson et al.
113: 1365
Supplemental materials for: Carlson et al
Files in this Data Supplement:
- Figure S1. TH17 cells, total T cells, or CD4+/CD25− cells induce little pathology in the kidney (JPG, 1.51 MB)
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5 × 106 TH17 cells (n=6), whole splenic T cells (n=4), or CD4+/CD25− (n=5) cells were transferred with T-cell–depleted bone marrow cells into lethally irradiated B6D2 recipients. At day 14 post-transplantation kidneys were harvested and processed as described in Materials and Methods. TT = total T cells, CD4 = CD4+/CD25− cells.
- Figure S2. Non-GFP+ T cells exhibit little autofluorescence (JPG, 776 KB)
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3 × 106 naïve whole T cells from WT C57BL/6 mice were transferred with T-cell–depleted bone marrow cells into lethally irradiated B6D2 recipients. Seven days after transfer animals were anesthetized with avertin and organs were imaged with a Zeiss SteREO Lumar.V12 microscope with eGFP bandpass filter. Brightfield images (left), and GFP images (middle) were taken for each organ. GFP intensities (right) were determined by software analysis. (A) liver (B) lung (C) spleen (D) mesenteric lymph node.
- Figure S3. A population of in vitro polarized TH17 cells produce IFN-γ in vivo (JPG, 724 KB)
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1.35 × 106 eGFP+ TH17 cells were transferred with T-cell–depleted bone marrow cells into lethally irradiated B6D2 recipients. Eight days post-transplant lymphocytes were extracted from the lung and stimulated as in Fig. 1 followed by intracellular cytokine staining for IFN-γ. Plot derived from eGFP+ / IL-17− gate.
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