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Blood, Vol. 113, Issue 10, 2202-2212, March 5, 2009 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef PIAS3 negatively regulates RANKL-mediated osteoclastogenesis directly in osteoclast precursors and indirectly via osteoblasts Blood Hikata et al. 113: 2202 Supplemental materials for: Hikata et al Files in this Data Supplement: Document 1. Supplemental materials and methods (PDF, 59.3 KB) Table S1. Primers (PDF, 758 KB) Figure S1. PIAS3 did not affect the cell proliferation and cell survival (JPG, 24.2 KB) - (A) Cell proliferation activity of mock- and PIAS3-transduced RAW264.7 cells was analyzed by a WST-1 assay. (B) Apoptosis of mock- and PIAS3-transduced RAW264.7 cells was measured by DNA fragmentation assay. Error bars in all panels represent SD. n.s, not significant. Figure S2. Downregulation of PIAS3 enhances RANKL-mediated osteoclastogenesis (JPG, 101 KB) - (A) Analysis of osteoclast differentiation by TRAP staining. RAW264.7 cells were transfected with 5nM or 10nM siRNA specific for negative control or PIAS3, and were stimulated by various concentrations of RANKL as indicated. (B) TRAP positive MNCs having more than 3 or 10 nuclei were counted as osteoclast. (C) BMMs transfected with negative control or PIAS3 siRNA were cultured for 5 days in the presence of M-CSF (30ng/ml) and RANKL (50ng/ml). Cultured cells were fixed and stained for TRAP. (D) TRAP positive MNCs having more than 3 nuclei were counted as osteoclast. Error bars in all panels represent SD. #,P<0.05; ##,P<0.01; n.s, not significant. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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