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Blood, Vol. 112, Issue 12, 4598-4608, December 1, 2008
Primary infection with simian immunodeficiency virus: plasmacytoid dendritic cell homing to lymph nodes, type I interferon, and immune suppression
Blood Malleret et al.
112: 4598
Supplemental materials for: Malleret et al
Files in this Data Supplement:
- Table S1. Monoclonal antibodies used for pDC phenotyping (PDF, 368 KB)
- Figure S1. Histological control staining (JPG, 77.4 KB)
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Serial lymph node sections (10 µm) were stained with DAPI and by immunofluorescent reagents, with isotype controls. Magnification: ×100.
- Figure S2. Correlations between different parameters, taking into account all the macaques studied (JPG, 91.5 KB)
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Spearman’s rank correlations between the area under the curve (AUC) of the different parameters calculated from day 0 to 21 post-infection are shown. Closed black symbols: macaques of the 5,000 AID50 group; closed gray symbols: macaques of the 50 AID50 group; open black symbols: macaques of the 50 AID50/ART group.
- Figure S3. Phenotypic characterization of cynomolgus macaque blood plasmacytoid dendritic cells (JPG, 90.4 KB)
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(A) Representative dot plot set showing lineage-negative (CD3, CD8, CD14, and CD20) HLA–DR-positive CD1c-negative CD123-positive cells (black dots) in whole blood: left panel, FSC/SSC scattergrams for ungated events; middle panel, HLA-DR/Lineage dot plot for G1-gated events; right panel, CD1c/CD123 dot plot for Lineage-negative HLA-DR–positive events, (B) histograms showing expression of CD4, CXCR4, CCR5, CD7, CD45RA, CD1c, CD11b, CD36, CD40, CD80, CD86, and CD206 on gated HLA-DR+CD123+ pDC representative of 3 uninfected macaques.
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