|
|
Blood, Vol. 113, Issue 14, 3182-3189, April 2, 2009
Abnormal megakaryocyte morphology and proplatelet formation in mice with megakaryocyte-restricted MYH9 inactivation
Blood Eckly et al.
113: 3182
Supplemental materials for: Eckly et al
Files in this Data Supplement:
- Figure S1. In situ immunofluorescence staining of bone marrow cells from WT and MYH9Δ mice (JPG, 62.7 KB)
-
Cryosections from WT (left) or MYH9Δ bone marrow (right) were double labeled with an anti-β3 integrin antibody (red) and an anti-VWF antibody (green). Megakaryocytic cells were examined by confocal microscopy. Bars: 75 µm.
- Figure S2. The residual normal and truncated forms of myosin IIA are abnormally distributed in MYH9Δ megakaryocytes (JPG, 32.5 KB)
-
(A) Myosin IIA distribution in megakaryocytes from MYH9Δ bone marrow (left panel, green labeling). Cryosections were labeled with an anti-myosin IIA antibody and an AF488-conjugated secondary antibody and examined by confocal microscopy. The image has been overexposed (as compared to Fig. 3A) to visualize the residual myosin which is clustered in the cytoplasm (arrows). The same section was co-labeled with an anti-β3 integrin antibody so that megakaryocytes were more easily recognizable (right panel, red labeling). (B) Western blot showing the presence of residual amounts of full-length myosin (arrowhead) and truncated myosin (arrow) in MYH9Δ megakaryocytes cultured for 4 days. Actin was visualized to check for identical protein loading between lanes. Images are representative of 2 independent experiments.
|
|
