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Blood, Vol. 113, Issue 13, 2976-2987, March 26, 2009
Gene expression profile of the third pharyngeal pouch reveals role of mesenchymal MafB in embryonic thymus development
Blood Sultana et al.
113: 2976
Supplemental materials for: Sultana et al
Files in this Data Supplement:
- Figure S1. Quantitative PCR analysis of total cellular RNA from indicated cell fractions purified from E14.5 C57BL/6 mice (JPG, 90.3 KB)
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mRNA levels of indicated genes were normalized to GAPDH mRNA levels, and are indicated as in Figure 3. Means and standard errors of three independent measurements are shown.
- Figure S2. Numbers of thymic stromal cells in MafB-deficient mice (JPG, 85.9 KB)
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E15.5 thymus lobes were digested and stained for CD45, PDGFRα, and IA. The numbers of CD45−PDGFRα+ thymic mesenchymal cells (Ms), CD45−IA+ thymic epithelial cells (Ep), and PDGFRα−IA− thymic stromal cells were calculated from total viable thymus cells and the frequencies of respective populations. Data are means and standard errors of three to four independent measurements. In all the three cell populations, cell numbers among MafB+∕+, MafB+∕−, and MafB−∕− mice were not significantly different (P > 0.05).
- Figure S3. Detection of Vγ3+ T cells in the thymus and the skin of E18.5 MafB-deficient embryos (JPG, 104 KB)
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(A, B) E18.5 fetal thymocytes isolated from indicated mice were stained for CD45, TCRδ, and either Vγ3 or Vγ2. Representative flow cytometry profiles of CD45+ cells are shown in panel A. Numbers in dot plots indicate frequency of cells within the quadrant. The numbers of indicated cells were calculated from the number of total viable thymocytes and the frequency of that population. Means and standard errors of three to four independent measurements are shown in panel B. (C) Representative immunofluorescence images of Vγ3+ cells (green) in the skin of E18.5 embryos. Scale bars indicate 50 µm. Representative results of 4–6 images are shown.
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