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Blood, Vol. 113, Issue 24, 6094-6101, June 11, 2009 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Human embryonic stem cells differentiate into a homogeneous population of natural killer cells with potent in vivo antitumor activity Blood Woll et al. 113: 6094 Supplemental materials for: Woll et al Files in this Data Supplement: Figure S1. Schematic representation of transposon and transposase vectors utilized to derive K562 cells stably expressing Firefly luciferase (JPG, 52.4 KB) - The bicistronic pKT2 vector consists of transposase binding sites (IR/DRs, filled triangles) that flank a fusion between GFP reporter and blasticidin (Bsd) resistance genes transcriptionally regulated by PGK promoter and firefly luciferase gene transcriptionally regulated a CpG-free promoter (CLP) consisting of a synthetic enhancer (en) and human elongation factor 1α promoter (EF1α). The transposase-encoding plasmid pPGK-SB11 was utilized to facilitate stable integration. Figure S2. Flow cytometric analysis of CD56+ sorted NK cells used for in vivo injection (JPG, 204 KB) - Cells cultured in NK cell conditions for 35 days were harvested and magnetically sorted for CD56+ cells. Greater than 99% purity was achieved for both hESC- and UCB-derived NK cells. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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