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Blood, Vol. 113, Issue 6, 1257-1267, February 5, 2009
Distinctive localization of antigen-presenting cells in human lymph nodes
Blood Angel et al.
113: 1257
Supplemental materials for: Angel et al
Files in this Data Supplement:
- Table S1. Summary of APCs in lymph nodes from different sites within each patient (PDF, 31.4 KB)
- Figure S1. CD1a+CD207+ APCs in human dermis co-express CD1b (JPG, 147 KB)
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Immunohistochemistry illustrated that CD1a+CD207+ cells in the dermis expressed CD1b (White arrow heads (A–D)). In contrast the CD1a+CD207+ epidermal Langerhans cells lacked CD1b expression (A–D). In the dermis CD207+ APCs were often associated with elongated cellular structures (E) that expressed the lymphatic vessel endothelial markers LYVE-1 (White arrow heads F–G) and podoplanin (White arrow heads H), however they were also sometimes remote from these structures (Yellow arrow heads I). Interestingly CD207+CD1b+ APCs co-expressed LYVE-1 (F–G), confirming expression of this marker by APCs (Bockle et al., 2008, Placenta 29:187). Epidermis is orientated upwards (A–D, F, and H–I) and its interface with the dermis is indicated by dotted line (A–D and F). Inserts show individual and merged expression of CD1a, CD207, and CD1b (A), and LYVE-1, CD1b, and CD207 (F) for the same area. White arrowheads in inserts point to the same cell (A and F). Blue represents DAPI staining of cell nuclei (E, H, and I). Data are representative of four independent experiments.
- Figure S2. APCs expressing a similar phenotype to cutaneous APCs were restricted to lymph nodes from particular sites (JPG, 102 KB)
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Immunohistochemistry was used to detect APCs expressing CD1a, CD207, CD208, CD209, and BDCA-2 in frozen sections of inguinal, cervical, and hilar lymph nodes, draining the skin, head, and neck area and lungs respectively. CD1a+CD207− APCs and CD1a+CD207+ APCs were detected in the inguinal lymph node (A) and one of the three hilar lymph nodes screened (D); the remaining two hilar and all cervical lymph nodes lacked cells expressing these markers (B–C). APCs expressing CD208 exhibited a similar distribution pattern; a number CD208+ APCs were detected in the inguinal lymph node (E) and one of the three hilar lymph nodes assessed (I) (interestingly this was the same hilar lymph node that contained CD1a+ and CD207+ cells). Very few CD208+ cells were detected in the remaining two hilar and cervical lymph nodes (F–H). In contrast CD209+ APCs were densely clustered in the medulla and sparsely scattered throughout all lymph nodes tested (E–I). Rare BDCA-2+ plasmacytoid APCs were detected in all the lymph nodes tested (J–L), although they were slightly more prevalent in the inguinal lymph node screened (J). Blue represents DAPI staining of cell nuclei. * indicates background auto-fluorescence. Seven lymph nodes were assessed, one inguinal, three cervical, and three hilar.
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