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Blood, Vol. 113, Issue 12, 2843-2850, March 19, 2009 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Transferrin-a modulates hepcidin expression in zebrafish embryos Blood Fraenkel et al. 113: 2843 Supplemental materials for: Fraenkel et al Files in this Data Supplement: Table S1. Sequences of morpholinos used to knockdown zebrafish transferrin-a or transferrin receptors (PDF, 401 KB) - Morpholinos for TfR1a, TfR1b, and GATA1 are as described.18,26 Mismatch substitutions are shown in lower-case. The sequence targeting an ATG is underlined. Figure S1. Co-injection of TfR1a cRNA partially rescues anemia in transferrin-a morphants prior to the onset of hepcidin expression (JPG, 55.2 KB) - O-dianisidine staining (anterior) for hemoglobin at 36 hpf comparing (A) uninjected WT, (B) morpholino knockdown of transferrin-a, (C) co-injection TfR1a cRNA with transferrin-a morpholino, (E) co-injection of TfR1a cRNA with transferrin-a morpholino. (D) In situ hybridization for hepcidin in uninjected WT (dorsolateral) at 36 hpf revealing that hepcidin expression is not yet detectable. The scale bar represents 200 µm (A–C) or 100 µm (D). This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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