SEARCH:   Advanced

Blood, Vol. 113, Issue 18, 4458-4467, April 30, 2009 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Host natural killer T cells induce an interleukin-4–dependent expansion of donor CD4+CD25+Foxp3+ T regulatory cells that protects against graft-versus-host disease Blood Pillai et al. 113: 4458 Supplemental materials for: Pillai et al Files in this Data Supplement: Figure S1. Transplant protocol using congenic markers to determine the source of CD4+CD25+Foxp3+ donor T cells seen in wild-type hosts given TLI/ATS conditioning and untreated donor bone marrow and splenocyte transplants (JPG, 104 KB) - Wild-type CD45.2 BALB/c hosts were given infusion of 50 × 106 whole bone marrow from CD45.2 C57BL/6 donors and 60 × 106 splenocytes from CD45.1 C57BL/6 donors. Panel shows representative FACS pattern showing CD45.1 versus orthogonal scatter of gated H-2Kb+CD4+CD25+Foxp3+ cells in the spleen of transplanted hosts at day 6. Figure S2. Chimeric donor Tregs are suppressors in CFSE MLR Assay and potent secretors of IL-10 (JPG, 104 KB) - Mean ± SD percentage CFSElow cells amongst gated normal C57BL/6 CD4+ responder cells in CFSE MLR assays with syngeneic background control C57BL/6 stimulators or with BALB/c or third-party C3H stimulators. Allogeneic cultures were set up with addition of sorted normal (UNT, untreated) or chimeric (TLI) H-2Kd−CD4+CD25+ cells (Treg). Means of 3 experiments, each with triplicate cultures using pooled CD4+CD25+ cells from 3–6 mice. Details of the determination of CFSElow cells are given in Methods. (B) Mean ± SD IFN-γ, IL-4, and IL-10 concentrations in 40-hour supernatants from H-2Kb+CD4+CD25+ spleen cells sorted from untreated wild-type C57BL/6 mice (UNT Treg) or at day 6 from wild-type BALB/c hosts given TLI/ATS and transplant from C57BL/6 wild-type donors (TLI Treg). U: unstimulated; S: stimulated with PMA/ionomycin; ND: not detected; *: less than 100 pg/mL. Results shown are combined from 2 experiments, each from 20–30 pooled wild-type or 3–4 pooled TLI/ATS conditioned transplanted hosts. Figure S3. Host IFN-γ and IL-10 are not required to augment donor Treg accumulation or to reduce donor CD8+ T-cell accumulation in the host spleen and colon (JPG, 128 KB) - TLI/ATS-conditioned IFN-γ−∕− and IL-10−∕− BALB/c hosts were given infusion of 50 × 106 whole bone marrow and 60 × 106 splenocytes from wild-type C57BL/6 donors. Panels show representative FACS pattern for CD4 versus Foxp3 among gated H-2Kb+CD4+ cells (left panels) and CD4 versus CD8 among gated H-2Kb+TCRαβ+ cells for spleen (middle panels) and colon (right panels) of transplanted hosts at day 6. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

	Copyright © 2009 by American Society of Hematology         Online ISSN: 1528-0020