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Blood, Vol. 113, Issue 13, 2999-3007, March 26, 2009
IL-7 sustains CD31 expression in human naive CD4+ T cells and preferentially expands the CD31+ subset in a PI3K-dependent manner
Blood Azevedo et al.
113: 2999
Supplemental materials for: Azevedo et al
Files in this Data Supplement:
- Figure S1. Adult naive CD31+ CD4+ T cells proliferate in response to IL-7 as measured by CFSE labelling (JPG, 26.9 KB)
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Naïve CD4+ T cells were purified from the peripheral blood by negative selection using the EasySep Human Naïve CD4+ T-Cell Enrichment Kit (Stem Cell Technologies) and stained with CFSE. Cells were cultured for 7 days in the presence or absence of IL-7. At Day 1 and at Day 7 cells were harvested and stained using monoclonal antibodies against CD4, CD45RA, and CD31. The percentage of CFSE negative was quantified following gating on CD4+CD45RA+ population and subsequent gating on CD31+ or CD31− subsets. Unstimulated cells were used as an additional control, and results obtained at Day 7 were similar to the ones at Day1 (data not shown).
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