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Blood, Vol. 113, Issue 7, 1483-1492, February 12, 2009
BLNK suppresses pre–B-cell leukemogenesis through inhibition of JAK3
Blood Nakayama et al.
113: 1483
Supplemental materials for: Nakayama et al
Files in this Data Supplement:
- Figure S1. Summary of karyotype analysis complemented with FISH (JPG, 57.2 KB)
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Representative HQ-banded karyotype from each BLNK-pBL was arranged. Each chromosome abnormality (exhibited in colored boxes) was confirmed by FISH analyses using chromosome specific painting probes. Cytogenetically, clonal expansion of the following karyotype for each cell lines was verified. BKO84: 41, X, +6, +10; DKO18: 41, XY, +mar (originated from chromosome 12); DKO35: 40, XX, dup(4)(pter→B3::D1→B3::B3→qter).
- Figure S2. Southern blot analysis of E2A and c-myc gene loci (JPG, 80.5 KB)
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Genomic DNA from indicated BLNK-pBLs as well as spleen from wild-type mouse were digested with the indicated restriction enzymes and subjected to Southern blot hybridization with the following probes: E12 cDNA fragment for E2A gene locus and genomic fragments spanning an exon1-intron1 region (probe 5′) or an exon2 region (probe 3′). Estimated lengths of the hybridizing bands are denoted under pictures representing each gene locus (closed box: exon).
- Figure S3. BLNK mRNA expression in pre-B leukemia cells of the BLNK+∕− STAT5b-CA mouse (JPG, 26.4 KB)
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Total RNAs from the same samples used in Fig. 3C, individual lymph nodes (LN1-5) in the BLNK+∕− STAT5b-CA mouse #1 and MACS-purified splenic B cells of BLNK+∕− mouse, were subjected to RT-PCR analysis to examine the expression levels of BLNK (top) and GAPDH (bottom, as a cDNA quantity control). For the PCR of BLNK cDNA, primers that amplify a full coding sequence were used.
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