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Blood, Vol. 113, Issue 26, 6695-6698, June 25, 2009
TGF-β induces degradation of TAL1/SCL by the ubiquitin-proteasome pathway through AKT-mediated phosphorylation
Blood Terme et al.
113: 6695
Supplemental materials for: Terme et al
Files in this Data Supplement:
- Figure S1. TAL1 is poly-ubiquitinylated (JPG, 175 KB)
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HeLa cells were transfected with 1 µg of pSGF-TAL1 and 0.5 µg of pSG-HA-Ub as indicated. Cell lysates were used for immunoprecipitation with an antibody to FLAG. Immunoblot analysis of cell extracts (lane 1 to 3) and immunoprecipitates (lanes 4 to 6) was done using antibodies to HA (top panel) and to FLAG (bottom panel). Positions of the TAL1 polyubiquitinated forms (bar on the right) and of FLAG-TAL1 are indicated.
- Figure S2. Subcellular localisation of CHIP and TAL1 in Jurkat cells (JPG, 63.9 KB)
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Jurkat cells were fractioned in nuclear (lane 1) and cytoplamic (lane 2) extracts which were analyzed by immunoblot using antibodies to TAL1 (top panel), to R2 (middle panel), used here as a control of a cytoplasmic protein, and to CHIP (bottom panel). Position of the signal corresponding to these three proteins is indicated on the right.
- Figure S3. E47 prevents TAL1 ubiquitinylation (JPG, 298 KB)
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HeLa cells were transfected with 1 µg of pSGF-TAL1, 1 µg of myc-E47, and 0.5 µg of pSG-HA-Ub as indicated. Cell lysates were used for immunoprecipitation with an antibody to FLAG. Immunoblot analysis of immunoprecipitates (lanes 1 to 6) and extracts (lanes 7 to 12) was done using antibodies to HA (top panel), to FLAG (middle panel), and to myc (bottom panel). Positions of signals corresponding to polyubiquitinylated TAL1 (bar on the right), FLAG-TAL1, and Myc-E47 are indicated.
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