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Blood, Vol. 113, Issue 14, 3218-3225, April 2, 2009 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Activin-A attenuates several human natural killer cell functions Blood Robson et al. 113: 3218 Supplemental materials for: Robson et al Files in this Data Supplement: Figure S1. Assessment of NK cell purity (JPG, 44.1 KB) - NK cells were stained immediately after MACS purification with antibodies against CD56 and CD3 and purity determined by flow cytometry. One representative donor is shown. These highly purified NK cells predominantly expressed intermediate levels of CD56 (CD56Int) but also contained a minor subpopulation of CD56Bright NK cells. Figure S2. Effects of TLR ligands on NK cell IFN-γ production and effects of activin-A on NK cell CD25 expression (JPG, 38.3 KB) - Purified NK cells were cultured at 1 × 105 in 96 well plates in media supplemented with IL-2 (20 U/ml) + IL-12 (10 ng/ml) with or without addition of TLR ligands as shown. (A) NK cell IFN-γ production as detected by ELISA at 24 h. (B) CD25 expression by NK cells following stimulation in the absence or presence of activin-A was determined by flow cytometry. Results from three donors are shown in (A) or data represents the mean ± 1SD of three separate donors in (B). *p<0.01 vs no activin-A. Figure S3. The effects of activin-A on NK cell proliferation (JPG, 74.8 KB) - Representative analysis of one of the three NK donors from the data set shown in Fig. 6 as FACS profiles. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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