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Blood, Vol. 113, Issue 14, 3209-3217, April 2, 2009
Identification of a particular HIV-specific CD8+ T-cell subset with a CD27+ CD45RO–/RA+ phenotype and memory characteristics after initiation of HAART during acute primary HIV infection
Blood Lécuroux et al.
113: 3209
Supplemental materials for: Lecuroux et al
Files in this Data Supplement:
- Table S1. Clinical and biological characteristics of the 35 subjects with primary HIV infection (PDF, 64.4 KB) -
* done at Month 3 (data not available at Day 0)
** done at Month 1 (data not available at Day 0)
- Figure S1. Reciprocal and exclusive expression of RA and RO isoforms of CD45 (JPG, 50.5 KB)
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PBMC were isolated from HAART-treated subjects and labeled with HIV-multimer-APC, CD45RA-FITC, CD27-PE, CD45RO-PECy7, CD8-PerCPCy5.5, and CD3-APC-AlexaFluor750. Figure A shows the exclusive and mutual expression of RA and RO isoforms of CD45. B and C display similar percentages of HIV-specific CD8+ T cells in the CD27+CD45RO− and CD27+CD45RA+ subsets; and in CD27+CD45RO+ and CD27+CD45RA− subsets. Representative phenotypes gated on HIV-specific CD8+ T cells from the same subject.
- Figure S2. HIV- and EBV-specific CD8+ T-cell characteristics in healthy and HIV-infected subjects (JPG, 60.8 KB)
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PBMC from 12 HIV-negative and 13 HIV-infected subjects were studied for EBV-specific responses (white) and compared with HIV-specific responses (grey). (A) PBMC were labeled with EBV- or HIV-multimer, CD27, CD45RO, and CD8 antibodies. The relative percentage of CD45RO−/RA+ in CD27+ EBV- or HIV-specific CD8+ T cells was calculated and is presented for each group of subjects. (B) PBMC were stained with CFSE and culture during 6 days with the relevant peptide. The percentages of EBV- or HIV-specific proliferating cells (CFSELow) are presented. (C-E) PBMC were stimulated overnight with the relevant peptide. The relative percentages of responding cells among EBV- or HIV-specific cells were calculated and are shown for IFN-γ, TNF-α and IL-2 production.
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