|
|
Blood, Vol. 113, Issue 15, 3612-3619, April 9, 2009
Paradoxical effects of IFN- in graft-versus-host disease reflect promotion of lymphohematopoietic graft-versus-host reactions and inhibition of epithelial tissue injury
Blood Wang et al.
113: 3612
Supplemental materials for: Wang et al
Files in this Data Supplement:
- Figure S1. Non–T-cell–derived IFN-γ fails to prevent GVHD (JPG, 41.1 KB)
-
(A) B6D2F1 mice received 6 Gy TBI one day before administration of purified WT (■) or GKO (▲) B6 T cells (7.5 × 106) plus T-cell–depleted (TCD) splenocytes (1.75 × 107) and BMCs (8 × 106) from WT B6 donors (n=5 per group). Mice receiving GKO T cells showed significantly greater body weight loss compared to those receiving WT T cells (p<0.005). (B) B6D2F1 mice received 6 Gy TBI one day before administration of purified WT (□) or GKO (△) B6 T cells (7.5 × 106) plus TCD splenocytes (1.75 × 107) and BMCs (8 × 106) from GKO donors (n=5 per group). Mice receiving WT T cells had significantly less weight loss than those receiving GKO T cells (p<0.05). The results shown in A and B were obtained from a single experiment and presented separately for the sake of clarity. The same non-HCT control group, i.e., B6D2F1 mice receiving TBI only (●/○; n=3), is presented in both figures.
- Figure S2. IFN-γ promotes the destruction of recipient hematopoietic cells by donor CD8 T cells (JPG, 23 KB)
-
B6D2F1 mice received 6 Gy TBI one day before administration of 6 × 106 allogeneic CD8 T cells alone (A) or along with 8 × 106 TCD BMCs (B) from WT (●) or GKO (○) B6 donors. Non-HCT controls were sublethally-irradiated B6D2F1 mice receiving no transplant. WBC were prepared at days 5 and 20, and percentages of host-type (H−2d) cells were determined by flow cytometry. The total number of host-type WBC was calculated as the product of the percentage of host-type cells and total white count. Shown are numbers (mean ± SDs) of host-type WBC at the indicated time points.
|
|
