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Blood, Vol. 113, Issue 13, 3050-3058, March 26, 2009
High-level expression of the T-cell chemokines CCL3 and CCL4 by chronic lymphocytic leukemia B cells in nurselike cell cocultures and after BCR stimulation
Blood Burger et al.
113: 3050
Supplemental materials for: Burger et al
Files in this Data Supplement:
- Table S1. Induction of CCL3 and CCL4 protein expression in CLL cell co-cultures with NLC (PDF, 13.3 KB) -
Displayed are the mean and SEM protein concentrations for CCL3 and CCL4 in supernatants from CLL-NLC co-cultures on days 2, 7, and 14 from 20 different patients. These data correspond to the data displayed in Fig. 2A.
- Table S2. CCL3 and CCL4 expression in CLL co-cultures with NLC (PDF, 31.7 KB) -
Displayed are the individual supernatant concentrations for CCL3 and CCL4 at days 2, 7, and 14 from 20 different patients. The means (±SEM) are at the bottom of the table. The patients were selected based upon their expression for ZAP-70, as determined by flow cytometry. Using a 20% cutoff to distinguish ZAP-70–negative from ZAP-70–positive patients, 10 patient samples were considered ZAP-70–positive, and 10 samples were ZAP-70–negative. The data displayed in this table correspond to the dot plots shown in Fig. 2B and 2C.
- Table S3. CCL3 protein expression in co-cultures of ZAP-70–positive (n=10) and ZAP-70–negative (n=10) CLL samples with NLC (PDF, 16 KB) -
Displayed are the mean (±SEM) CCL3 concentrations in supernatants for these different patient groups on days 2, 7, and 14. CCL3 concentrations were higher in ZAP-70–positive samples at all timepoints, but due to individual variations, statistical significance was not reached; the p values are displayed on the right hand side. These data correspond to the bar diagrams in Fig. 3A.
- Table S4. CCL4 protein expression in co-cultures of ZAP-70–positive (n=10) and ZAP-70–negative (n=10) CLL samples with NLC (PDF, 16 KB) -
Displayed are the mean (±SEM) CCL4 concentrations in supernatants for these different patient groups on days 2, 7, and 14. CCL4 concentrations were higher in ZAP-70–positive samples at all timepoints, but due to individual variations, statistical significance was not reached; the p values are displayed on the right hand side. These data correspond to the bar diagrams in Fig. 3B.
- Table S5. CLL patients' characteristics (PDF, 32.2 KB) -
Displayed are the key clinical characteristics of the 20 CLL patients that were studied for CCL3 and CCL4 protein induction in NLC co-cultures.
- Table S6. CCL3 protein expression after CLL cell stimulation for 24h with different concentrations of anti-IgM, anti-CD40, or medium alone (PDF, 17.9 KB) -
Displayed are the CCL3 concentrations for 3 individual patients, and the mean and standard deviations, corresponding to the data displayed in Fig. 5A.
- Table S7. CCL4 protein expression after CLL cell stimulation for 24h with different concentrations of anti-IgM, anti-CD40, or medium alone (PDF, 17.9 KB) -
Displayed are the CCL4 concentrations for 3 individual patients, and the mean and standard deviations, corresponding to the data displayed in Fig. 5B.
- Table S8. CCL3 and CCL4 protein expression after CLL-NLC co-culture for 24h and 48 hours with or without the Syk inhibitor R406 (PDF, 26.5 KB) -
Displayed are the individual CCL3 and CCL4 concentrations for 4 different CLL patients, and the mean and standard deviations, corresponding to the data displayed in Fig. 7.
- Figure S1. Correlation between induced CCL3 (A) and CCL4 (B) expression (CCL3/CCL4 expression in CLL-NLC co-cultures minus baseline CCL3/CCL4 expression on day 0) and ZAP-70 expression (mean ZAP70 mRNA expression, log2 transformed, in NLC co-cultures) (JPG, 50.5 KB)
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The dots represent in this scatterplot the individual expression levels for the 9 different patients tested, and the regression line indicates the relationship between both variables. Moreover, the Pearson correlation coefficient and the p value are displayed in the top left hand corner.
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