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Blood, Vol. 114, Issue 1, 187-194, July 2, 2009
SCF induces  -globin gene expression by regulating downstream transcription factor COUP-TFII
Blood Aerbajinai et al.
114: 187
Supplemental materials for: Aerbajinai et al
Files in this Data Supplement:
- Figure S1. Phenotyping studies of erythroid progenitor cells cultured in EPO and EPO plus SCF on day 14 (JPG, 103 KB)
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CD34+ cells cultured in EPO-containing medium for 6 d were stimulated with or without SCF (50 ng/mL) from day 6–14. Cytospins prepared on days 14 cells were stained with May-Grunwald Giemsa to identify the cell types for morphological analyses, and cells were labeled with fluorescein isothiocyanate (FITC)-conjugated antibodies directed against glycophorin A (GPA) for phenotyping. (A) Representative fields of Giemsa-stained erythroid cells in EPO only or EPO plus SCF. (B) Flow cytometric analyses of cells stained with anti-glycophorin A (GPA) antibody. The percentage in the right upper quadrant of each panel describes the population staining positive for GPA. These data are representative of 3 independent experiments.
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