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Blood, Vol. 113, Issue 4, 963-972, January 22, 2009
CX3CR1 is required for monocyte homeostasis and atherogenesis by promoting cell survival
Blood Landsman et al.
113: 963
Supplemental materials for: Landsman et al
Files in this Data Supplement:
- Figure S1. Differential effect of enforced Bcl2 expression on the two monocyte subsets (JPG, 56.2 KB)
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(A) Expression of hBcl2 transgene by monocyte subsets. MACS purified CD115+ blood cells from cx3cr1gfp∕+;hMRP8bcl2 and cx3cr1gfp∕+ littermates were stained with anti-Gr1 and anti-human Bcl2 antibodies. Dot plot shows Gr1hi (R7 gated cell) and Gr1low (R8 gated cell) monocyte populations. Histograms show hBcl2 expression by the subsets of hMRP8bcl2 transgenic (empty histogram) and non-transgenic (gray filled histogram) mice. (B) Comparison of blood monocyte populations of wt (black filled circles) and hMRP8bcl2 (empty circles) mice. Diagram shows the numbers of total Gr1hi and Gr1low monocytes (identified as indicated in Figure 1A) in 1 ml of blood. Note that bcl2 expression rescues more Gr1low than Gr1hi monocytes.
- Figure S2. Reduced atherogenesis in the absence of CX3CR1 (JPG, 37.4 KB)
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Analysis of atherosclerotic lesion area in apoe−∕− recipients of wt, cx3cr1gfp∕+ sand cx3cr1gfp∕gfp BM cells, Atherosclerotic plaques were analyzed in the aorta after Oil-red-O staining. (A) Aorta of representative mouse for each group. Titles indicate BM donor genotype. (B) Quantitative analysis of apoe−∕− recipients of wt (empty circles), cx3cr1gfp∕+ (gray circles) and cx3cr1gfp∕gfp (black circles) BM cells. n=5. *pValue<0.01, ns=non significant (Student’s t-Test).
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