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Blood, Vol. 113, Issue 24, 6112-6119, June 11, 2009
Plasmacytoid dendritic cells accumulate in spleens from chronically HIV-infected patients but barely participate in interferon- expression
Blood Nascimbeni et al.
113: 6112
Supplemental materials for: Nascimbeni et al
Files in this Data Supplement:
- Table S1. Antibodies used for flow cytometry (PDF, 28 KB)
- Table S2. Antibodies used for in situ immunofluorescence (PDF, 30.6 KB)
- Figure S1. Isotype controls and DAPI staining from the HIV− and HIV+ patients (JPG, 2.68 MB)
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DAPI stainings were done on spleen serial spleen sections from the same patients as shown in figs. 1 and 3. Staining of serial spleen sections from HIV− patients with cancer (A, left panel, control for Fig. 1A left panels), ITP (A, right panel, control for Fig. 1A right panels; B, right panel) and from HIV+ patients with ITP (B, left panel, patient M, control for Fig. 3A left panels; C, patient S, control for Fig. 3C; D, patient R, control for Fig. 3D iv). Panel A shows mouse IgG2a and IgG1 isotype controls for ASM and CD123 labeling (controls for Fig. 1A). Panels B and C shows mouse IgG2a and IgG1 isotype controls for ASM and BDCA2 labeling (controls for figs. 3A and 3C). Panel D shows rabbit IgG and mouse IgG1 controls for interferon and BDCA2 staining from patient R (control for Fig. 3D iv). Original magnification: ×100.
- Figure S2. IFN-α intracellular labeling in pDC characterized using either CD123- or BDCA4-specific antibodies (JPG, 104 KB)
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Data are from patient M as in Fig. 4 and are representative of 4 HIV+ patients.
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