|
|
Blood, Vol. 113, Issue 3, 733-743, January 15, 2009
Host CD4+CD25+ T cells can expand and comprise a major component of the Treg compartment after experimental HCT
Blood Bayer et al.
113: 733
Supplemental materials for: Bayer et al
Files in this Data Supplement:
- Figure S1. Residual host Treg cells comprise the majority of the regulatory T-cell compartment following treatment with busulfan and/or cyclophosphamide (JGP, 103 KB)
-
C57BL/6 mice were treated for 4 consecutives days with 20mg/kg busulfan and/or on the fourth day treated with a single dose of 200mg/kg of cyclophosphamide. PBS-treated mice served as controls. On the second day following treatment, all mice received 10 × 106 TCD-BM from B6-Thy1.1 congenic mice. Four weeks later, all mice were sacrificed and the thymus, spleen and lymph node from individual mice were subjected to flow cytometry analysis. (A) Representative histogram showing Thy1.1 (donor BM) staining from the thymus. (B) Representative dot plots showing CD4 and Foxp3 staining with corresponding dot plot illustrating Thy1.1 (donor BM) and Thy1.2 (host) staining of the gated CD4+Foxp3+ population in the spleen (left panels) and lymph nodes (right panels). Data in (A) and (B) are representative of 3–4 mice per group.
|
|
