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Blood, Vol. 113, Issue 21, 5352-5360, May 21, 2009
Inhibition of endothelial progenitor cell differentiation by VEGI
Blood Tian et al.
113: 5352
Supplemental materials for: Tian et al
Files in this Data Supplement:
- Figure S1. Histograms of flow cytometry analysis of EPC markers on EPC cultured for 7 days in the absence (white curves) or presence of VEGI (shaded curves) (JPG, 69.1 KB)
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We determined the effect of VEGI treatment on the expression of nine typical surface markers of EPC as the cells differentiate into endothelial cells in culture. Six endothelial markers Flk-1, Tie-2, E-selectin, VE-cadherin, CD105, and CD31, and three stem cell markers Sca-1, AC133, and CD117 were examined by flow cytometry. Bone marrow-derived EPC retained stem cell markers and prominently expressed endothelial cell markers after 7 days in culture. In comparison, cells cultured in the presence of VEGI otherwise under identical conditions expressed much lower levels of endothelial cell markers. However, the expression levels of the stem cell markers in VEGI-treated cells were similar to those in the untreated EPC.
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