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Blood, Vol. 113, Issue 17, 3961-3968, April 23, 2009 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Novel function for interleukin-7 in dendritic cell development Blood Vogt et al. 113: 3961 Supplemental materials for: Vogt et al Files in this Data Supplement: Table S1. Primary reagents used for flow cytometry (PDF, 73.9 KB) Table S2. Secondary reagents used for flow cytometry (PDF, 9.94 KB) Table S3. Antibodies used for in vitro blocking experiments (PDF, 45.4 KB) Table S4. Reagents used for immunofluorescence (PDF, 52.3 KB) Figure S1. Gating strategy for pDCs in mixed bone marrow chimeras (JPG, 103 KB) - pDCs isolated from pLN (A) and spleen (B) of mixed bone marrow chimeras were stained with antibodies and identified by flow cytometry as follows: gate on small cells and exclusion of doublets (FSC-A versus FSC-W), with cells being CD11b− and CD11cint Gr-1+. pDCs identified by this strategy also express B220 (histogram). Figure S2. BrdU incorporation is similar in IL7Rα+∕+ and IL-7Rα−∕− DCs (JPG, 65.8 KB) - Lethally irradiated CD45.1/.2+ mice were reconstituted with an equal mixture of WT BM (CD45.1+) and IL-7Rα−∕− BM (CD45.2+). 16 weeks later mice were fed with BrdU water for 3 days before analysis by 6-color flow cytometry. (A, B) cDCs (CD11c+ MHCII+) and migDCs (CD11c+ MHCIIhi) from pLNs were stained with an antibody to BrdU (black) or an isotype-matched control antibody (grey) (n=3–5). Representative histograms (A) and bar graphs (B) depicting the percentage of BrdU+ DCs in spleen and pLN are shown. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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