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Blood, Vol. 113, Issue 18, 4197-4205, April 30, 2009 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Concentration-dependent inhibition of angiogenesis by mesenchymal stem cells Blood Otsu et al. 113: 4197 Supplemental materials for: Otsu et al Files in this Data Supplement: Figure S1. Characterization of MSC (JPG, 54.4 KB) - Flow cytometric analysis of culture-expanded rat MSC was performed as detailed in the Methods section. The fraction of positive events is indicated in those analyses that had detectable marker expression. Specific antibody profiles (shaded histograms) versus isotype-matched control (line histogram) are shown. n=3 (each group). Figure S2. RLMEC capillaries on day 11 (JPG, 43.6 KB) - Images show RLMEC capillaries after 11 days of seeding in Matrigel. Left image is phase contrast. Right image shows GFP fluorescence. Bar=500 µm. All imaging data replicated at least six times. Figure S3. Cx43 expression in RLMEC and MSC (JPG, 32.3 KB) - Images show Cx43 expression in RLMEC (left) and rat MSC (right) monolayers. Immunocytochemistry was carried out with a monoclonal Cx43 primary antibody and Alexa 543 conjugated secondary antibody. Bar=25 µm. All imaging data replicated at least six times. Figure S4. Fluorescence recovery after photobleaching (FRAP) in Cx43T154A expressing EC capillaries (JPG, 28.4 KB) - Left: plot shows FRAP time course in Cx43 mutant (Cx43mt) or empty vector (VEC) transfected RLMEC capillaries. Group data show fluorescence recovery 20 min after bleaching n=6 (each group). Mean ± SD. *P<0.05 against empty vector. Figure S5. Capillary formation in Cx43T154A-expressing EC (JPG, 32.3 KB) - Phase contrast (left) and fluorescence (right) images show day-old EC capillaries expressing GFP-Cx43T154A. Left is phase contrast image. Right is GFP fluorescence image. Bar=500 µm. All imaging data replicated at least six times. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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