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Blood, Vol. 113, Issue 16, 3875-3884, April 16, 2009
Breaking tolerance to self, circulating natural killer cells expressing inhibitory KIR for non-self HLA exhibit effector function after T cell–depleted allogeneic hematopoietic cell transplantation
Blood Yu et al.
113: 3875
Supplemental materials for: Yu et al
Files in this Data Supplement:
- Figure S1. Comparison of overall functional responsiveness of the complete NK cell repertoire post-HSCT to donor repertoire (JPG, 21.4 KB)
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Percentage of IFN-γ+ cells from donor-patient NK repertoires (n=16 pairs) in response to coincubation with 721.221. The response of the total NK cell repertoire in the early post-HSCT period is slightly lower than donor repertoire response (P=.06).
- Figure S2. NK cells expressing 3DL1 from an individual with Bw4 exclusively contributed by HLA-A2402 are functionally competent (JPG, 79.6 KB)
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The percentage of IFN-γ–producing cells among KIR-expressing NK populations from the HLA-C1/C2, Bw4/Bw6 donor, the HLA-identical patient (Patient #15) pre-transplant, and the patient at day +30, day +60, and day +100 post-HSCT following co-incubation with the indicated target cells. The Bw4 epitope is contributed solely by the HLA-A2402 antigen. NK cells exclusively expressing KIR2DL1 (A), 2DL3 (B), and 3DL1 (C) exhibit comparable effector capacity, higher responsiveness immediately post-HSCT, and ability to be inhibited by the appropriate cognate class I ligand.
- Figure S3. KIR−NKG2A+ NK cells display lower effector potential following HSCT (JPG, 22.6 KB)
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Percentage of IFN-γ-producing cells among the NK cells exclusively expressing CD94/NKG2A upon exposure to 721.221 is shown. NK cells from the normal healthy donors and the patients (#10, #12, and #14) pre-transplant, at day +30, day +100, and at day +200 post-HSCT show an early decrease in effector function among cells exclusively expressing CD94/NKG2A (*P=.046). The percentage of IFN-γ–producing cells was calculated by KIR−NKG2A+IFN-γ+/KIR−NKG2A+.
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