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Blood, Vol. 113, Issue 19, 4711-4719, May 7, 2009
CXCR4 is a key regulator of neutrophil release from the bone marrow under basal and stress granulopoiesis conditions
Blood Eash et al.
113: 4711
Supplemental materials for: Eash et al
Files in this Data Supplement:
- Figure S1. Morphologically mature neutrophils are present in the bone marrow of MKO mice (JPG, 103 KB)
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(A) Representative micrographs of bone marrow cytospins from two different MKO mice or a wild type (WT) mouse demonstrating the presence of band and segmented neutrophils. Original magnification ×1000. Images were acquired using an E plan 100×/1.25 NA oil immersion objective, Microphot SA microscope, Digital Sight DS-Fi1 camera, and NIS-Elements F2.30 software, all from Nikon (Melville, NY). (B) Manual leukocyte differential counts were performed on control (CTL) or MKO mice. Data represent the mean ± SEM (n = 3 mice per group).
- Figure S2. MKO mice have normal numbers of myeloid progenitors in the bone marrow (JPG, 46.4 KB)
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Bone marrow cells from control (CTL) or MKO mice were plated in methylcellulose medium in the presence of stem cell factor, IL-3, IL-6, and EPO (CFU-C) or G-CSF (CFU-G) and colonies enumerated on day 7–10 of culture. Data represent the mean ± SEM (n = 5 mice per group).
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