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Blood, Vol. 114, Issue 2, 357-359, July 9, 2009
Endogenous IL-17 contributes to reduced tumor growth and metastasis
Blood Kryczek et al.
114: 357
Supplemental materials for: Kryczek et al
Files in this Data Supplement:
- Table S1. Immune cell subsets in wild versus IL-17–deficient mice with or without tumor (PDF, 18.4 KB)
- Figure S1. IL-17 has no effect on MC38 apoptosis, cell cycle and proliferation (JPG, 119 KB)
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MC38 cells (1 × 105/ml) were cultured with or without IL-17 (10 ng/ml) for 72 hours. Apoptosis (A), cell cycle (B) and proliferation (C, D) of MC38 were analyzed. Four independent experiments were realized. (A) Apoptosis. MC38 cells were stained with Annexin V and PI, and analyzed with FACS. Results were expressed as the percentages of apoptotic MC38 cells. (B) Cell cycle. Four hours after BrDU incorporation, MC38 cells were stained for 7-AAD and BrDU, and analyzed with FACS. Results were expressed as the percentages of MC38 cells in each phase of cell cycle. (C, D) Cell proliferation. (C) MC38 cells were stained for Ki67, and analyzed by FACS. Results were expressed as the percentages of Ki67+ MC38 cells in total viable cells. (D) four hours after addition of 3Hthymidine, thymidine incorporation was measured. Results were expressed as the mean c.p.m ± SD. P > 0.05.
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