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Blood, Vol. 113, Issue 18, 4250-4261, April 30, 2009
Limited ability of humoral immune responses in control of viremia during infection with SIVsmmD215 strain
Blood Gaufin et al.
113: 4250
Supplemental materials for: Gaufin et al
Files in this Data Supplement:
- Document 1. Supplemental materials and methods (PDF, 67.6 KB)
- Figure S1. The neutralization sensitivity of seven primary SIVsmm isolates belonging to different phylogenetic lineages (JPG, 82 KB)
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Strains belonging to 5 different lineages (lineage 1: SIVsmmM919, SIVsmmM935; lineage 2: SIVsmmM926 and SIVsmmM946; lineage 3: SIVsmmM951; lineage 4: SIVsmmG932 and lineage 6: SIVsmmD215 were tested. Neutralization sensitivity was evaluated using sera from sooty mangabeys chronically infected with SIVsmm strains belonging to different phylogenetic lineages (lineage 1: M919, M935, M947; lineage 2: M926, M946; lineage 3: A023, M941, M951; Lineage 4: G930, G931, G932; lineage 5: D175, F098 and lineage 6: D215). The X-axis shows the susceptibility of different strains to neutralization by autologous and heterologous sera. The Y-axis displays the neutralizing titers (in logs).
- Figure S2. Alignment of CD16-derived amino acid sequences obtained by cloning and sequencing rhesus macaque cDNA from whole blood (JPG, 418 KB)
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Amino acid differences between the reference RM sequence and AGM sequences are illustrated. The mature peptide begins at residue 18, as indicated by the arrow. Two vertical arrows indicate the boundaries of exon S2. Potential N-glycosylation sites and cysteines involved in disulfide bonds are bolded. Shaded amino acids indicate amino acids in human CD16 that interact with human Ig1. Protein domains are indicated by arrows. Mamu-Macaca mulatta; Mafa-Macaca fascicularis; Hu-humans.
- Figure S3. Assessment of viral trapping (JPG, 193 KB)
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Viral trapping in LN sampling (A) and Peyer’s patches (C) of Rituximab-infused RMs with complete CD20+ B-cell depletion, compared to control RMs (B and D). Both LN sampling and intestinal resections were performed at day 28 p.i. Magnification: 20×. Germinal centers are marked by circles.
- Figure S4. Changes in CD4+ T cells subsets in blood (A, C, E) and intestine (B, D, F) in rituximab-infused RMs with complete CD20 depletion (■), incomplete tissue CD20 depletion (▼) and in control RMs (●) (JPG, 114 KB)
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Dynamics of naïve (CD3+ CD4+ CD28+ CD95neg) (A and B), central memory (CD3+ CD4+ CD28+ CD95+) (C and D) and effector memory (CD3+ CD4+ CD28neg CD95+) (E and F) is presented. Plots represent the average expression for the animals in each study group. Vertical lines represent the SEM.
- Figure S5. Peripheral blood SIV-specific cellular immune responses, as assessed by the IFN-g ELISPOT assay with peptide pools spanning the entire SIVsmm proteome (JPG, 128 KB)
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ELISPOT responses (in stacked columns) are presented in relation to SIVsmm RNA plasma VLs (black lines).SIV proteins are color-coded, as follows: Gag-red, Pol-mustard, Env-orange, Tat-dark green, Rev-yellow, Nef-blue, Vpr-pink, Vpx-light green, Vif-violet.
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