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Blood, Vol. 113, Issue 17, 4114-4124, April 23, 2009
Long-term outcome after hematopoietic stem cell transplantation of a single-center cohort of 90 patients with severe combined immunodeficiency
Blood Neven et al.
113: 4114
Supplemental materials for: Neven et al
T-cell and B-cell immune function assessment
The total peripheral blood lymphocyte count and various lymphocyte subsets were evaluated using flow cytometry (CD3+, CD3+CD4+, CD3+CD8+, as well as CD19/CD20+ B cells, CD3−CD16+ NK cells). Since 2000, we have also analyzed naive CD31+CD45RA+ T-cell counts within the CD3+CD4+ subset as an indicator of the naive T-cell population; this parameter was found to correlate strongly with the determination of T-cell receptor excision circles (+).28 In vitro lymphocyte proliferation induced by mitogens and antigens was evaluated by 3Hthymidine uptake. The serum levels of IgG, IgA and IgM were measured by nephelometry. Serum antibodies to polioviruses and tetanus and diphtheria toxoids were measured using enzyme-linked immunosorbent assays after booster immunization in some patients.
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