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Blood, Vol. 113, Issue 9, 1909-1918, February 26, 2009
Human erythrocytes bind and inactivate type 5 adenovirus by presenting Coxsackie virus-adenovirus receptor and complement receptor 1
Blood Carlisle et al.
113: 1909
Supplemental materials for: Carlisle et al
Files in this Data Supplement:
- Figure S7. Erythrocytes from CAR transgenic mice express CAR and those from CR1 transgenic mice express CR1, whereas WT mice express neither (JPG, 50 KB)
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Flow cytometry was performed as described for figure 1B, staining with isotype control (black line), anti-CAR antibody RmcB (red line) or anti-CR1 antibody (green line).
- Figure S8. Erythrocyte ghost membranes present CAR and CR1 (JPG, 20.2 KB)
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Erythrocyte ghosts were formed as described in methods and then tested for presentation of CAR and CR1 using flow cyotmetry. (A) shows staining with isotype control (black line) or anti-CAR antibody RmcB (red line). (B) shows staining with isotype control (black line) or anti-CR1 (green line).
- Figure S9. Binding of Ad5 to erythrocytes as visualized by cryoEM (JPG, 48.5 KB)
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Erythrocyte ghosts were prepared as described in methods and exposed to Ad5 in PBS or in a 1 in 100 dilution of fresh human plasma, before preparation for and visualisation by CryoEM. (A) shows some representative binding events for human erythrocytes in PBS, (B) shows human erythrocytes in human plasma, (C) shows erythrocytes from CAR transgenic mice in PBS and (D) shows the absence of binding to erythrocytes from CR1 transgenic mice in PBS.
- Figure S10. Ad5 surface charge alteration as a result of polymer coating (JPG, 32.8 KB)
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The surface charge of Ad5 or EGF-p-Ad5 was (109 copies) was measured using a Zetasizer 3000 HS (Malvern Instruments). The mean and standard error of 10 runs is shown.
- Figure S11. Comparison of normal and EGF-mediated infection in neat neutralising serum (JPG, 23.2 KB)
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Ad5 or EGF-P-Ad5 were incubated with neat neutralising antisera and then added to a monolayer of A431 cells, after 90 minutes media was removed and washing performed in PBS and after 24 hours luciferase expression analyzed.
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