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Blood, Vol. 114, Issue 2, 404-414, July 9, 2009
MicroRNA profiling identifies miR-34a and miR-21 and their target genes JAG1 and WNT1 in the coordinate regulation of dendritic cell differentiation
Blood Hashimi et al.
114: 404
Supplemental materials for: Hashimi et al
Files in this Data Supplement:
- Table S1. Complete miRNA expression and statistics (XLS, 408 KB)
- Table S2. MiRNA target lists generated using either Sanger MirBase (miRanda), TargetScan, or Pictar target prediction algoritms (XLS, 659 KB)
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Reduced target gene list (in alphabetical order) was generated using Part I of the target ranking system (i.e., targets predicted by two or more of the three target prediction algorithms).
- Table S3. Unique targets of increasing/decreasing miRNAs upon MDDC differentiation (XLS, 41 KB)
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List generated from Part II of the target ranking system. These final lists were used for GOstat functional analysis.
- Table S4. GOstat functional ontology results of target gene list generated from Part II of increasing miRNA group (XLS, 1.29 MB)
- Table S5. GOstat functional ontology results of target gene list generated from Part II of decreasing miRNA group (XLS, 223 KB)
- Table S6. Reduced GOstat functional ontology results of target gene list generated from Part II of decreasing miRNA group (XLS, 186 KB)
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GOstat functional ontology results that were also found in the group of increasing miRNA analysis were eliminated from this list.
- Figure S1. qRT-PCR confirms expression patterns of twelve miRNAs in MDDC differentiation (JPG, 171 KB)
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qRT-PCR was performed on three independent donors (one representative donor shown) at indicated timepoints using miRNA-specific primers and probes. Error bars represent standard deviation of three replicates. All values were normalized to RNU43 small nuclear RNA as an endogenous control.
- Figure S2. Schematic flow diagram of our two part target ranking system (JPG, 326 KB)
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Part I generates a reduced target list for each miRNA, Part II culls and prioritizes the targets for functional validation. See text for detailed rationale.
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