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Blood, Vol. 113, Issue 21, 5202-5205, May 21, 2009
Reduction of T cell–derived ghrelin enhances proinflammatory cytokine expression: implications for age-associated increases in inflammation
Blood Dixit et al.
113: 5202
Supplemental materials for: Dixit et al
Files in this Data Supplement:
- Figure S1 (JPG, 28.0 KB)
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(A) Acylated ghrelin inhibits inflammatory cytokine expression from human T cells. Peripheral human T cells were activated via immobilized anti-CD3 antibody in presence or absence of ghrelin (100ng/ml) and/or the GHS-R antagonist, D-Lys-3-GHRP-6 (DLS, 10−4 M) for 48 hours. The harvested supernatants were subsequently assayed for IL-6, TNFα, and IL-17. A representative experiment of three separate donors is shown, and the data is expressed as the mean ± SD. Ghrelin significantly inhibited the expression of IL-6, TNFα, and IL-17 in the culture supernatants when compared to the control cells (*P < 0.05). In addition, pretreatment with DLS significantly abrogated the ghrelin inhibitory effects when compared to ghrelin-treated cells alone (*P < 0.05). DLS alone failed to significantly influence cytokine expression in these cultures. (B) Densitometric analysis of the gel bands of the total protein lysates from control and ghrelin siRNA transfected cells were analyzed for phosphorylation of IkB and demonstrated a greater than 2-fold increase in pIkB levels when compared to control siRNA-transfected cells. Moreover, control siRNA transfected cells were treated with 100ng/ml of ghrelin for 5 minutes demonstrated a greater than 4-fold decrease in pIkB levels when compared to control siRNA-transfected cells.
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