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Blood, Vol. 113, Issue 23, 5979-5998, June 4, 2009
Changes in gene expression of granulocytes during in vivo granulocyte colony-stimulating factor/dexamethasone mobilization for transfusion purposes
Blood Drewniak et al.
113: 5979
Supplemental materials for: Drewniak et al
Files in this Data Supplement:
- Table S1 (PDF, 42.3 KB) -
Genes, which expression was similarly affected after in vivo and in vitro treatment with G-CSF- dexamethasone, were analyzed by the OntoExpress program (http://vortex.cs.wayne.edu/projects.htm#Onto-Express), and categorized into proper functional profiles (e.g. transcription, signal transduction, immune response, cell faith & apoptosis, cell adhesion & mobility, metabolism). Only selection of genes falls into those categories. Results are presented as the mean fold-increase or –decrease of three separate donors (comparison is with freshly isolated neutrophils t=0).
- Table S2. Gene sequence data (XLS, 170 KB)
- Figure S1. Scheme of the experiment (JPG, 76.4 KB)
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- Figure S2. Regulation of calpastatin levels in neutrophils (JPG, 177 KB)
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(A) Samples of neutrophils cultured in the presence or absence of G-CSF and dexamethasone were taken at the indicated times and analyzed by Western blot. Where indicated, CHX (10 µg/mL) was added to prevent new protein synthesis. Shown is a representative blot from 1 out of 5 independent experiments. (B) Samples of neutrophils cultured in the presence or absence of Calpain Inhibitor-3 (CI3, 20 µM) or the pan-caspase inhibitor zVAD (20 µM) were taken at the indicated time points and analyzed by Western blot. Shown is a representative blot from 1out of 5 independent experiments.
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