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Blood, Vol. 114, Issue 2, 444-451, July 9, 2009
Treatment of invasive streptococcal infection with a peptide derived from human high-molecular weight kininogen
Blood Oehmcke et al.
114: 444
Supplemental materials for: Oehmcke et al
Files in this Data Supplement:
- Figure S1. In vivo and in vitro testing of the antimicrobial effect of HKH20 (JPG, 165 KB)
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(A) Neutropenic mice were infected i.p. with 5 × 106 CFUs S. pyogenes, in the absence or presence of HKH20 or aprotinin. 10 h after infection mice were sacrificed, spleens were removed and CFUs in the spleens determined. (B) Incubation of 106 CFUs S. pyogenes with 200 µg/ml HKH20 (0,323 mM) for 2 hours at 37°C (in vitro). (C) Mice were infected i.p. with 5 × 106 CFUs S. pyogenes, in the presence or absence of HKH20 and spleens processed after 18 h of infection.
- Figure S2. Clearance of HKH20 from murine blood (JPG, 76.7 KB)
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Mice were injected i.p. with 200 µg Alexa Fluor®555-labeled HKH20 for 15, 30, 60, or 120 min. Plasma samples were collected and analyzed spectroscopically for their fluorescence. As controls served: PBS, plasma from a non-treated mouse (ctrl.), and plasma from a mouse injected i.p. with dye only (15 min).
- Figure S3. Fluorescence microscopy pictures of the organs (JPG, 378 KB)
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Mice were injected i.p. with 200 µg Alexa Fluor®555-labeled HKH20 for 15, 30, 60, or 120. Animals were sacrificed and main organs (lung, spleen, liver, and kidney) were removed and investigated by fluorescence microscopy (×60). Scale bar represents 100 µm.
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