|
|
Blood, Vol. 114, Issue 1, 95-104, July 2, 2009
KIR acquisition probabilities are independent of self-HLA class I ligands and increase with cellular KIR expression
Blood Andersson et al.
114: 95
Supplemental materials for: Andersson et al
Files in this Data Supplement:
- Table S1. HLA genotype of donors (PDF, 42.9 KB) -
BC, Buffy Coat; BC320 was B13/Bw6 and therefore not considered to have a strong ligand for KIR3DL1.
- Figure S1. Observed frequencies of NK cells co-expressing four KIRs are not due to spectral overlap of multiple flourochromes (JPG, 236 KB)
-
(A) NK cells expressing four inhibitory KIRs pre and post sorting. (B) Sorted NK cells were cultured in IL-2 for 5 days cells and stained with the indicated antibodies one by one (solid line) or with all mAbs simultaneously (dotted line). Remaining fluorescence from the pre-sort staining with the indicated mAbs is represented by shaded area.
- Figure S2. Pascal’s triangle-like representation of sequential KIR acquisition (JPG, 114 KB)
-
Depicted are the possible ways for NK cells to acquire KIRs in a sequential manner where Xij is a random variable corresponding to i successes and j failures. Each checkpoint corresponds to a binomial outcome with probability p for success and q=1-p for failure. The solid red line represents the path for calculating the probability that a given NK cell succeeds in expressing three KIRs in a row and then fails to express the fourth KIR at the fourth check-point (X30) to give X31. In this case, the probability is given by p00*p10*p20*q30. The overall probability of expressing three KIRs is obtained by summing the probabilities given by each possible path (solid plus dotted lines) leading to X31.
- Figure S3. Characteristics of the three identified KIR repertoires (JPG, 107 KB)
-
The frequency of NK cells expressing the indicated receptors in low, intermediate and high KIR repertoires. KIRavg represent the average KIR expression and was given by dividing total KIR expression frequencies by four.
- Figure S4. KIR expression is independent of cognate HLA ligands (JPG, 95.8 KB)
-
The observed frequencies of NK cells were compared to those predicted from a sequential selection model. Frequencies of NK cells expressing zero to three KIRs in individuals with one (n=18), two (n=16), and three (n=6) strong KIR ligands excluding KIR3DL2 and HLA-A3/-A11 as a functional interaction.
- Figure S5. Modeling KIR repertoires under the influence of selection by cognate HLA class I molecules (JPG, 206 KB)
-
(A) Schematic illustration of the algorithm used to calculate the probabilities for expression of self (ps) and nonself (pns) KIRs. The algorithm takes all possible combinations of KIRs and KIR ligands into consideration. (B) An example of how KIR acquisition is stopped in a given NK cell that acquires a self KIR. The solid red line indicates the path for expressing two KIRs, one of which is a self KIR. The probability for this event is given by pns00*ps10*q20*q21. After acquisition stop q equals 1.
|
|
