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Blood, Vol. 113, Issue 18, 4370-4380, April 30, 2009
PDLIM2 suppresses human T-cell leukemia virus type I Tax-mediated tumorigenesis by targeting Tax into the nuclear matrix for proteasomal degradation
Blood Yan et al.
113: 4370
Supplemental materials for: Yan et al
Files in this Data Supplement:
- Figure S1. Effect of Tax on PDLIM2 mRNA expression (JPG, 50 KB)
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(A) Immunoblotting assays for the induction of Tax expression in Jurkat-Tet-Tax (Jurkat-Tax) by tetracycline (Tet). (B) Real-time PCR analysis for the RNA expression of PDLIM2 was analyzed in the Jurkat-Vector or Jurkat-Tax cells treated with tetracycline for the indicated times. The relative levels of PDLIM2 mRNA were normalized according to β-actin mRNA level and represented as percentile in untreated Jurkat-Vector cells (arbitrarily set as 100). The data presented are the mean ± standard deviation (n = 3). (C) Comparison of PDLIM2 RNA in the MEF cells stably expressing Tax or an empty vector by real-time PCR described in B.
- Figure S2. PDLIM2 expression had no obvious effect on degradation of Tax-activated p65 (JPG, 68.7 KB)
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The indicated cells expressing exogenous PDLIM2 or an empty control vector were chased as the indicated time points, followed by subcellular fractionation. The insoluble nuclear fractions, which is the degradation site of activated p65 (Tanaka et al., 2007), were used for IB to detect expression levels of endogenous p65. In the lanes 15 and 18, the cells were chased in the presence of MG132.
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