|
|
Blood, Vol. 113, Issue 19, 4604-4613, May 7, 2009
Mantle cell lymphoma cells express high levels of CXCR4, CXCR5, and VLA-4 (CD49d): importance for interactions with the stromal microenvironment and specific targeting
Blood Kurtova et al.
113: 4604
Supplemental materials for: Kurtova et al
Files in this Data Supplement:
- Table S1. Phenotype of CD5+ B cells derived from normal donors (PDF, 13.2 KB) -
Surface expression of CD49d, CXCR4, and CXCR5 on CD5+ B cells, presented as mean fluorescence intensity ratios (MFIR). Data shown are the mean ± SEM of 4 experiments with CD5+ B cells from different donors.
- Figure S1. Expression of CD49d, CXCR4, and CXCR5 on normal CD5+ B cells (JPG, 71.3 KB)
-
(A) Contour plot that depicts the staining and gating of mononuclear cells to determine CD49d, CXCR4, and CXCR5 expression on normal CD5+ B cells. At least 5000 CD5+ B cells were acquired within the gate that defines CD5+ B cells, based upon CD19 and CD5 co-expression. (B) Histogram overlays show the expression of CD49d, CXCR4, and CXCR5 (red histogram) on CD5+ B cells compared with the respective isotype control (blue histograms), and the MFIR for each molecule is displayed next to the histogram. The figures shows representative plots from 1 out of 4 experiments with normal CD5+ B cells from different donors.
- Figure S2. Chemotaxis and lack of chemokinesis in MCL cells in response to CXCL12 and CXCL13 (JPG, 59.1 KB)
-
Displayed are the mean ± SEM values of 3 different experiments with SP-53 (A) or MINO (B) to distinguish chemotaxis from chemokinesis. Stimulation of both SP-53 and MINO cells resulted in a robust migration towards CXCL12 (200 and 500 ng/ml) and CXCL13, provided that the chemokines were placed into the lower chamber to establish a chemokine gradient. However, if the chemokines were placed at the same concentration into both chambers, as indicated by “chemokinesis” on the left hand side of each diagram, the MCL did not display significant migration. These findings indicate that CXCL12 and CXCL13 induce directional MCL cell migration (chemotaxis), but they do not induce random migration (chemokinesis). As internal controls within the assays, MCL were also pre-incubated with Plerixafor or pertussis toxin prior to migration towards CXCL12. The asterisks indicate significant differences in inhibition of chemotaxis compared to control with p values that are < 0.05.
|
|
