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Blood, Vol. 114, Issue 2, 478-484, July 9, 2009
The prototype endothelial marker PAL-E is a leukocyte trafficking molecule
Blood Keuschnigg et al.
114: 478
Supplemental materials for: Keuschnigg et al
Files in this Data Supplement:
- Figure S1. Analysis of expression levels of PV-1 in vim−∕− and wild type mice (JPG, 225 KB)
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Exemplary figures from the heart are depicted. Upper panel shows the original figures acquired from the section and lower panel shows excised vascular areas on which
the analysis was performed. Quantification of expression levels was objectively carried out by comparing fluorescence pixel intensity-values using the built in histogram-function of the Zeiss LSM-software and student’s T-test function in Excel.
- Figure S2. PV-1 partially colocalizes with vimentin in the cell periphery upon TNF-α activation (JPG, 491 KB)
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(A) Magnification of the area of co-localization from Fig. 1C. (B) Another cell, showing a limited co-localization of PV-1 and vimentin.
- Figure S3. Analysis of whole-blood samples (JPG, 47.9 KB)
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In order to rule out induction of leucopenia by administered antibodies, whole-blood samples were collected and values analyzed. (A) Blood samples from peritonitis and (B) from air pouch model. WBC, white blood cells.
- Video 1. Time-lapse confocal microscopy of a lymphocyte migrating through a HUVEC (AVI, 8.67 MB)
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The movie shows the opening of a transcellular pore in front of an extravasating lymphocyte. Fig. 3A is depicted from this video.
- Video 2. 3D reconstruction of the channel from Fig. 3A and Video 1 reaching through the endothelial cell (AVI, 5.96 MB)
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The reconstruction was made from a stack of confocal z-sections.
- Video 3. Maximum projection showing 2 lymphocytes transcellularly migrating through a vascular HDMEC (AVI, 4.95 MB)
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The 3D reconstruction was carried out using a Z stack consisting of 12 sections spaced 0,45 µm.
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