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Blood, Vol. 113, Issue 25, 6342-6350, June 18, 2009
Prospective isolation and molecular characterization of hematopoietic stem cells with durable self-renewal potential
Blood Kent et al.
113: 6342
Supplemental materials for: Kent et al
Files in this Data Supplement:
- Table S1. Tags over-represented in the adult BM HSC library vs. the FL HSC library (PDF, 135 KB)
- Table S2. Tags over-represented in the FL HSC library vs. the adult BM HSC library (PDF, 93.3 KB)
- Figure S1. LongSAGE libraries prepared from HSC-enriched fractions of E14.5 FL and adult BM cells (JPG, 751 KB)
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(A) Scatter plot of individual tags and their respective counts in LongSAGE libraries prepared from HSC-enriched fractions of adult BM (Y-axis) and FL (X-axis). Confidence intervals of 95, 99, and 99.9% were determined using Audic Claverie statistics.27 (B) Summary of the size and content of each library. (C) Gene Ontology analysis of significantly over-represented tags in the adult BM library (blue) as compared to the FL library and vice versa (red). (D) Normalized number of tags per 105 total tags present in each library for genes expected to be present. N.D. = not detectable.
- Figure S2. Q-RT-PCR analyses of transcript levels of selected genes in various FL and adult BM cell populations as indicated (JPG, 854 KB)
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(A) Transcript levels of all genes assayed in the original screen for genes commonly upregulated in HSCs vs. lin− adult BM cells (blue bars = HSCs, white bars = lin−) and FL cells (red bars = HSCs, white hatched bars = lin−). (B) Transcript levels in the adult BM E-SLAM cells (blue bars) and CD45+EPCR+CD48−CD150− cells (green bars). (C) Transcripts levels in E-SLAM cells cultured in 20 ng/mL of IL-11 supplemented with either 300 ng/mL SF (dark purple bars), 10 ng/mL SF factor (intermediate purple bars) or 1 ng/mL SF (light purple bars). All values were normalized to Gapdh. Values shown are the mean ± SEM of values obtained in 2–6 independent experiments, with each measurement being derived from triplicate assays.
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