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Blood, Vol. 114, Issue 9, 1776-1783, August 27, 2009
Programming tumor-reactive effector memory CD8+ T cells in vitro obviates the requirement for in vivo vaccination
Blood Klebanoff et al.
114: 1776
Supplemental materials for: Klebanoff et al
Files in this Data Supplement:
- Figure S1. In vitro programming of TEM cells causes cells to execute an in vivo proliferative response leading to a similar degree of lymph node expansion and pattern of tissue partitioning as vaccine stimulated TEM cells (JPG, 144 KB)
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(A) Proliferative response of programmed TEM cells compared with vaccine stimulated and non re-stimulated TEM cells in secondary lymphoid tissues. Sublethally irradiated WT mice received non re-stimulated pmel TEM cells (○), pmel TEM cells programmed in vitro with plate bound anti-CD3/anti-CD28 for 24h prior to cell transfer (♦), or pmel TEM cells plus rFPgp100 vaccination (■). All treated mice received exogenous rIL-2. The percentage of adoptively transferred pmel-1 cells (identified as CD8+Vβ13+ lymphocytes/lymphocyte gate) were enumerated from a pooled collection of inguinal, axillary, and mesenteric lymph nodes of treated animals as a function of time. Each data point represents the average ± SEM of 3 independent mice per treatment group. (B) Tissue partitioning of in vitro programmed versus vaccine-stimulated pmel TEM cells. Sublethally irradiated WT mice received pmel TEM cells programmed in vitro with plate bound anti-CD3/anti-CD28 for 24h prior to cell transfer (♦) or pmel TEM cells plus rFPgp100 vaccination (■). All treated mice received exogenous rIL-2. Data are presented as the ratio of percentages of adoptively transferred pmel-1 cells enumerated from the spleens and pooled lymph nodes of individual animals in each stimulation group as a function of time. Each data point represents the average ± SEM of 3 independent mice per treatment group. P > 0.5 by Wilcoxon rank sum test.
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