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Blood, Vol. 113, Issue 26, 6611-6618, June 25, 2009
V 1 T lymphocytes producing IFN- and IL-17 are expanded in HIV-1–infected patients and respond to Candida albicans
Blood Fenoglio et al.
113: 6611
Supplemental materials for: Fenoglio et al
Files in this Data Supplement:
- Figure S1. Ex vivo CD4+ T cells of HIV-1 patients do not express IFN-γ nor IL-17 (JPG, 8.72 KB)
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PBMC from HIV-1 infected Pts (n=30) were stained with the PerCP–anti-CD3 and the FITC-conjugated anti-CD4 mAbs; cytoplasmic staining with PE-anti–IFN-γ or APC-anti–IL-17 was performed immediately after isolation (ex vivo) or after 4h of culture with PMA and ionomycin. Samples were analyzed by FACSCanto flow cytometer (Becton Dickinson). Results are expressed as percentage of IFN-γ+ or IL17+ cells, among CD3/CD4 gated cells as indicated, before and after stimulation; mean±SD from 30 Pts: * p<0.01 vs ex vivo.
- Figure S2. Production of IFN-γ and IL-17 in γδ T cells in response to PMA and ionomicin (JPG, 21.1 KB)
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PBMC from HIV-1 infected Pts (n=30) or Hds (n=10) were stained with the PerCP–anti-CD3 and the FITC-conjugated A13 or BB3 mAbs to identify Vδ1 or Vδ2 T cells respectively; cytoplasmic staining with PE-anti–IFN-γ (panel A) or APC-anti–IL-17 (panel B) was performed immediately after isolation (ex vivo) or after 4h of culture with PMA and ionomycin.. Samples were analyzed by FACSCanto flow cytometer (Becton Dickinson). Results are expressed as percentage of IFN-γ+ (panel A) or IL-17+ (panel B) in Vδ1 (black columns) or Vδ2 (white columns) T cells before and after stimulation; mean±SD from 30 Pts and 10 Hds. Panels A and B: * p<0.01 vs Hds; Panel A:** p<0.01 vs ex vivo.
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