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Blood, Vol. 114, Issue 3, 619-629, July 16, 2009
RhoA and Rac1 GTPases play major and differential roles in stromal cell–derived factor-1–induced cell adhesion and chemotaxis in multiple myeloma
Blood Azab et al.
114: 619
Supplemental materials for: Azab et al
Files in this Data Supplement:
- Table S1. Expression of RhoA, Rac1, and CDC42 detected by flow cytometry and shown as MFI (JPG, 33 KB)
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- Table S2. The effect of Y27632 and NSC23766 on the expression of CXCR4, LFA1, and VLA4, detected by flow cytometry and shown as MFI (JPG, 20.6 KB)
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- Table S3. Expression of ICAM and VCAM on BM stromal cells detected by flow cytometry and shown as MFI (JPG, 7.57 KB)
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- Table S4. Expression of ICAM and VCAM on BM stromal cells detected by flow cytometry and shown as MFI (JPG, 11.5 KB)
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- Figure S1. The effect of increasing doses of ROCK and Rac1 inhibitors and their combination on the survival of MM cells from cell lines and patient samples after treatment of 3 and 24hrs (JPG, 53.9 KB)
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Also see Video 1.
- Figure S2. Mouse skull anatomy defining the different niches of BM used for in vivo confocal imaging (JPG, 24.4 KB)
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- Video 1. Combination of ROCK and Rac inhibitors (AVI, 8.4 MB)
- Video 2. Control (AVI, 8.07 MB)
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Videos demonstrating the changes of cell motility and shape induced by SDF1 (which was added at 15sec), and the effect of the inhibitors on response of MM1S to SDF1. Treatment with ROCK inhibitor, but not Rac1 inhibitor, prevented cell-shape change and cell-motility induced by SDF1 in MM1S cells.
- Video 3. ROCK inhibitor (AVI, 8.31 MB)
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Videos demonstrating the changes of cell motility and shape induced by SDF1 (which was added at 15sec), and the effect of the inhibitors on response of MM1S to SDF1. Treatment with ROCK inhibitor, but not Rac1 inhibitor, prevented cell-shape change and cell-motility induced by SDF1 in MM1S cells.
- Video 4. Rac inhibitor (AVI, 6.48 MB)
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Videos demonstrating the changes of cell motility and shape induced by SDF1 (which was added at 15sec), and the effect of the inhibitors on response of MM1S to SDF1. Treatment with ROCK inhibitor, but not Rac1 inhibitor, prevented cell-shape change and cell-motility induced by SDF1 in MM1S cells.
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