Blood, Vol. 114, Issue 9, 1820-1830, August 27, 2009
ID1 promotes expansion and survival of primary erythroid cells and is a target of JAK2V617F-STAT5 signaling Blood Wood et al. 114: 1820 Supplemental materials for: Wood et al Files in this Data Supplement: Figure S1. Chromatin immunopreciptiation assays performed in HEL cells in the presence of the JAK2 kinase inhibitor AT9383 or vehicle control and analyzed by q-RT-PCR at the ID1 +5.5 STAT5 binding site and control sites 1Kb upstream and downstream (JPG, 75.8 KB) - Figure S2. Proportion of in vitro cutured E14.5 lineage negative fetal liver cells in each of the five stages of erythroid differentiation (R1-R5; as illustrated in Fig. 2A) 24 hours after retroviral trans¬duction with expression constructs for Jak2, Jak2V617F, or the empty vector (MIG) (JPG, 91.2 KB) - Figure S3. Western blot for Id1 and Cox IV expression in NIH-3T3 cells, 48 hours after retroviral transduction with ID1 siRNA knockdown constructs or a control construct targeting firefly luciferase (JPG, 61.6 KB) - Figure S4. Cellular DNA-content analyzed using propidium iodide in permeabilised lineage negative fetal liver cells transduced with retroviral control or Id1 knockdown constructs and cultured for 48 hours (JPG, 103 KB) - Permeabilisation of the cells permits quantification of a sub-G1 population representing apoptotic cells. Figure S5. Representative FACS plots for Annexin V and 7AAD staining in lineage negative E14.5 fetal liver cells cultured in vitro under low (0.01U/ml) erythropoietin concentrations for 48 hours following retroviral transduction with an Id1 expression construct or the empty vector (MIG) (JPG, 131 KB) - Figure S6. Comparision of the effect of retroviral overexpression of Jak2 V617F and Jak2 K539L in lineage negative murine E14.5 fetal liver cells on Id1 expression after 24 hours of in vitro culture, as compared to overexpression of the empty expression plasmid (MIG) (JPG, 70.3 KB) -
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