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Blood, Vol. 114, Issue 10, 2193-2196, September 3, 2009
Genetic evidence for a predominant role of PI3Kβ catalytic activity in ITAM- and integrin-mediated signaling in platelets
Blood Canobbio et al.
114: 2193
Supplemental materials for: Canobbio et al
Files in this Data Supplement:
- Figure S1. Analysis of the expression of PI3Kγ, PI3Kβ, and p85 in PI3KγKD and PI3KβKD platelets (JPG, 31.2 KB)
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Platelets from wild type (WT, black columns), PI3KγKD (γKD, white columns) and PI3KβKD (βKD, gray columns) mice were lysed, and equal amounts of proteins were separated on a 7.5% acrylamide gel, transferred to nitrocellulose, and probed with antibodies against p100γ, p110β, or p85. Band intensity was quantified by densitometric scanning, and the results are reported in the histogram. The expression of each protein in wild type platelets was arbitrary considered as 1. Platelets from all the three genotypes expressed comparable amount of p85. In PI3KγKD the expression of PI3Kβ catalytic subunit (p110β) was comparable to that seen in wild type platelets, and PI3KβKD platelets expressed normal levels of PI3Kγ catalytic subunit p110γ. The level of expression of the catalytic inactive p110γ in PI3KγKD platelets and p110β subunits and PI3KβKD platelets, respectively, was significantly reduced to about 40%, indicating a currently unexplained chemical instability of the mutated proteins. Results are the mean ± S.D of three different determinations.
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