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Blood, 1 July 2002, Vol. 100, No. 1, pp. 268-275
PHAGOCYTES
Expression and subcellular localization of NRAMP1 in
human neutrophil granules
François Canonne-Hergaux,
Jero Calafat,
Etienne Richer,
Mathieu Cellier,
Sergio Grinstein,
Neils Borregaard, and
Philippe Gros
From the Department of Biochemistry, Center for the
Study of Host Resistance, McGill Cancer Center, McGill University,
Montreal, QC, Canada; the Division of Cell Biology, The Netherlands
Cancer Institute, Amsterdam, Netherlands; INRS-Institut
Armand-Frappier, Laval, QC, Canada; the Division of Cell Biology, The
Hospital for Sick Children, Toronto, Canada; and the Department of
Hematology, University of Copenhagen, Copenhagen, Denmark.
Mutations at the Nramp1 gene cause susceptibility to
infections with intracellular pathogens. In human blood,
polymorphonuclear (PMN) leukocytes are the most abundant site of
NRAMP1 messenger RNA (mRNA) expression, suggesting that
NRAMP1 plays an important role in the activity of these cells. By
Northern blot analysis, NRAMP1 mRNA was only detected in
most mature neutrophils from bone marrow (band and segmented cells). A
high-affinity polyclonal rabbit antihuman NRAMP1 antibody directed
against the amino terminus of the protein was produced and used to
study cellular and subcellular localization of the protein in primary
human neutrophils. Subcellular fractionation of granule populations
together with immunoblotting studies with granule-specific markers
indicate that NRAMP1 expression is primarily in tertiary granules.
These granules are positive for the matrix enzyme gelatinase and the
membrane subunit of the vacuolar H+/ATPase and can be
recruited for exocytosis by treatment of neutrophils with phorbol
myristate acetate. Immunogold studies by cryoelectron microscopy with
primary neutrophils confirm that a majority (75%) of NRAMP1-positive
granules are also positive for gelatinase, but they also suggest
further heterogeneity in this granule population. Presence of NRAMP1 in
tertiary granules is in agreement with the late-stage appearance of
NRAMP1 mRNA during neutrophil maturation in bone marrow.
Finally, immunofluorescence studies of Candida albicans-containing phagosomes formed in neutrophils
indicate that NRAMP1 is recruited from tertiary granules to the
phagosomal membrane on phagocytosis, supporting a role for NRAMP1 in
the antimicrobial defenses of human neutrophils.
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