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Blood, 15 November 2002, Vol. 100, No. 10, pp. 3536-3544
HEMATOPOIESIS
hnRNP-K and Pur act together to repress the
transcriptional activity of the CD43 gene
promoter
Nicolas Da Silva,
Ajit Bharti, and
Carl S. Shelley
From the Renal Unit, Massachusetts General Hospital,
Harvard Medical School, Charlestown, and the Dana-Farber Cancer
Institute, Boston, MA.
CD43 is an abundant, heavily glycosylated molecule expressed
specifically on the surface of leukocytes and platelets. When leukocytes are at rest, CD43 acts to prevent both homotypic and heterotypic interactions. However, during leukocyte activation CD43
expression is repressed, facilitating the intercellular contact required for chemotaxis, phagocytosis, aggregation, adhesion to endothelium, and transendothelial migration. Consequently, CD43 repression plays a vital role both in innate and acquired immunity. Here we report that a dramatic down-regulation of CD43 mRNA levels occurs during activation of the leukocytic cell line K562. This repression coincides with repression of the transcriptional activity of
the CD43 gene promoter. We have determined that
heterogeneous nuclear ribonucleoprotein K (hnRNP-K) and Pur act
together to mediate repression of the CD43 promoter during
K562 activation. The hnRNP-K molecule and Pur bind single-stranded
DNA. Therefore, exposure of single-stranded structures within the
CD43 promoter probably plays a major role in effecting
CD43 repression.
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